Aldolase C Recombinant Rabbit Monoclonal Antibody [PSH01-82]
cat.: HA721734
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Aldolase C aa 50-100. |
| Positive control: | Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | cytoskeleton, cytosol, extracellular exosome, extracellular region |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:5,000 |
| Uniprot #: | SwissProt: P09972 Human | P05063 Mouse | P09117 Rat |
| Alternative names: | ALDC ALDO C aldoc ALDOC_HUMAN Aldolase 3 Aldolase C Fructose bisphosphate Brain type aldolase Brain-type aldolase Fructoaldolase C Fructose 1 6 biphosphate triosephosphate lyase Fructose bisphosphate aldolase C Fructose-bisphosphate aldolase C |
Images
|
Fig1:
Western blot analysis of Aldolase C on different lysates with Rabbit anti-Aldolase C antibody (HA721734) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Mouse heart tissue lysate (low expression) Lane 4: Mouse pancreas tissue lysate (negative control) Lane 5: Rat brain tissue lysate Lane 6: Rat hippocampus tissue lysate Lane 7: Rat heart tissue lysate (low expression) Lane 8: Rat pancreas tissue lysate (negative control) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aldolase C antibody (HA721734) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721734) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aldolase C antibody (HA721734) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721734) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (negative control) with Rabbit anti-Aldolase C antibody (HA721734) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721734) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.