PSMD7 Recombinant Rabbit Monoclonal Antibody [PSH01-83]
cat.: HA721735
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH01-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSMD7 aa 1-200 / 324. |
| Positive control: | 293, NIH/3T3, K562, Hela. |
| Subcellular location: | Cytosol, extracellular exosome, extracellular region, nucleoplasm, nucleus. |
| Recommended Dilutions:
IF-Cell |
1:200 |
| Uniprot #: | SwissProt: P51665 Human | P26516 Mouse |
| Alternative names: | 26S proteasome non ATPase regulatory subunit 7 26S proteasome non-ATPase regulatory subunit 7 26S proteasome regulatory subunit RPN8 26S proteasome regulatory subunit S12 integration site gene, mouse, homolog of Moloney leukemia virus 34 proviral Moloney leukemia virus 34 proviral integration MOV34 Mov34 homolog Mov34 protein homolog MOV34L P40 Proteasome (prosome, macropain) 26S subunit, non ATPase 7 Proteasome (prosome, macropain) 26S subunit, non ATPase, 7 (Mov34 homolog) Proteasome 26S S12 Proteasome 26S subunit, no-ATPase, 7 Proteasome subunit p40 PSD7_HUMAN PSMD 7 PSMD7 Rpn8 S12 |
Images
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Fig1:
Immunocytochemistry analysis of 293 cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of K562 cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA721735) at 10 μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.