Phospho-PDGFR beta (Y1009) Recombinant Rabbit Monoclonal Antibody [JE46-72]
cat.: HA721736
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE46-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Tyr1009 of human PDGFR beta. |
| Positive control: | NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate, SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate, C6 treated with 100ng/mL PDGF for 5 minutes cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Lysosome lumen. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P09619 Human | P05622 Mouse | Q05030 Rat |
| Alternative names: | Beta platelet derived growth factor receptor Beta-type platelet-derived growth factor receptor CD 140B CD140 antigen-like family member B CD140b CD140b antigen IBGC4 IMF1 JTK12 OTTHUMP00000160528 PDGF R beta PDGF Receptor beta PDGF-R-beta PDGFR 1 PDGFR PDGFR beta PDGFR1 PDGFRB PGFRB_HUMAN Platelet derived growth factor receptor 1 Platelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptide |
Images
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Fig1:
Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa (Glycosylation) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa (Glycosylation) Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.