Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]
cat.: HA721737
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE46-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human MST1 protein. |
| Positive control: | Ramos treated with 1μM Staurosporine for 1 hour cell lysate, NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate, C6 treated with 1μM Staurosporine for 3 hour cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13043 Human | Q13188 Human | Q9JI11 Mouse | Q9JI10 Mouse | O54748 Rat Entrez Gene: 65189 Rat |
| Alternative names: | DKFZp686A2068 FLJ90748 KRS1 KRS2 Mammalian STE20 like protein kinase 1 Mammalian STE20 like protein kinase 2 MST 1 MST 2 MST1 MST2 Serine/threonine kinase 3 (STE20 homolog yeast) Serine/threonine kinase 3 (Ste20 yeast homolog) Serine/threonine kinase 3 Serine/threonine kinase 4 Serine/threonine protein kinase 3 Serine/threonine protein kinase 4 Serine/threonine protein kinase Krs 1 Serine/threonine protein kinase Krs 2 STE20 like kinase MST1 STE20 like kinase MST2 STK 3 STK 4 STK3 STK4 YSK3 |
Images
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Fig1:
Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Ramos treated with 1μM Staurosporine for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 1μM Staurosporine for 3 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.