IL17A Receptor Recombinant Rabbit Monoclonal Antibody [JE35-89]
cat.: HA721738
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE35-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IL17A Receptor aa 801-850 / 866. |
| Positive control: | THP-1 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, Raji cell lysate, HL-60 cell lysate, HeLa. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q96F46 Human |
| Alternative names: | CANDF5 CD217 CD217 antigen CDw217 CTLA8 HIL 17R hIL17R I17RA_HUMAN IL 17 receptor A IL 17 receptor IL 17RA IL-17 receptor A IL-17RA IL17 IL17A receptor IL17R IL17RA IMD51 Interleukin 17 receptor A Interleukin-17 receptor A MGC10262 |
Images
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Fig1:
Western blot analysis of IL17A Receptor on different lysates with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: HL-60 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 96 kDa Observed band size: 160 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721738) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling IL17A Receptor with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.