IL17A Receptor Recombinant Rabbit Monoclonal Antibody [JE35-89]
cat.: HA721738
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE35-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 96 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human IL17A Receptor aa 801-850 / 866.
Positive control: THP-1 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, Raji cell lysate, HL-60 cell lysate, HeLa.
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: Q96F46 Human
Alternative names: CANDF5 CD217 CD217 antigen CDw217 CTLA8 HIL 17R hIL17R I17RA_HUMAN IL 17 receptor A IL 17 receptor IL 17RA IL-17 receptor A IL-17RA IL17 IL17A receptor IL17R IL17RA IMD51 Interleukin 17 receptor A Interleukin-17 receptor A MGC10262
Images
HA721738_1.jpg Fig1: Western blot analysis of IL17A Receptor on different lysates with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Raji cell lysate
Lane 6: HL-60 cell lysate

Lysates/proteins at 25 µg/Lane.

Predicted band size: 96 kDa
Observed band size: 160 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721738) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721738_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling IL17A Receptor with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.