HOXB4 Recombinant Rabbit Monoclonal Antibody [JE36-65]
cat.: HA721739
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE36-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HOXB4 aa 11-60 / 251. |
| Positive control: | Jurkat cell lysate, HL-60 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse embryo cartilage tissue, HL-60. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P17483 Human | P10284 Mouse Entrez Gene: 497988 Rat |
| Alternative names: | Homeo box 2F Homeo box B4 Homeobox 2F Homeobox B4 Homeobox protein Hox B4 Homeobox protein Hox-2.6 Homeobox protein Hox-2F Homeobox protein Hox-B4 Homeobox protein HoxB4 HOX 2 Hox 2.6 Hox 2F HOX B4 HOX2 Hox2.6 Hox2F HOXB 4 hoxb4 HXB4_HUMAN |
Images
|
Fig1:
Western blot analysis of HOXB4 on different lysates with Rabbit anti-HOXB4 antibody (HA721739) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lane 3: PC-12 cell lysate Lane 4: Mouse liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 28 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721739) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue with Rabbit anti-HOXB4 antibody (HA721739) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721739) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of HL-60 cells labeling HOXB4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721739, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.