CrkL Recombinant Rabbit Monoclonal Antibody [JE50-21]
cat.: HA721740
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE50-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CrkL aa 254-303 / 303. |
| Positive control: | K-562 cell lysate, A431 cell lysate, HT-29 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue, A431. |
| Subcellular location: | Cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P46109 Human | P47941 Mouse | Q5U2U2 Rat |
| Alternative names: | Crk L Crk like protein Crk-like protein Crkl CRKL_HUMAN Crkol HGNC:2363 Oncogene CrkL V crk avian sarcoma virus CT10 oncogene homolog like v crk sarcoma virus CT10 oncogene homolog (avian) like V crk sarcoma virus CT10 oncogene homolog avian like |
Images
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Fig1:
Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA721740) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: C6 cell lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721740) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (HA721740) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721740) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of A431 cells labeling CrkL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.