TIMP1 Recombinant Rabbit Monoclonal Antibody [JE59-72]
cat.: HA721741
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE59-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein within human TIMP1. |
| Positive control: | HT-29 cell lysate, PANC-1 cell lysate, SW1990 cell lysate, human pancreas tissue, HT-29. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P01033 Human |
| Alternative names: | Clgi Collagenase inhibitor Collagenase inhibitor, Human EPA EPO Erythroid Potentiating Activity Erythroid-potentiating activity Fibroblast collagenase inhibitor FLJ90373 HCI Human Collagenase Inhibitor Metalloproteinase inhibitor 1 Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214 TIMP 1 TIMP TIMP metallopeptidase inhibitor 1 TIMP-1 Timp1 TIMP1 protein TIMP1_HUMAN Tissue Inhibitor of Metalloproteinase 1 Tissue inhibitor of metalloproteinases 1 Tissue inhibitor of metalloproteinases Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor |
Images
|
Fig1:
Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (HA721741) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721741) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP1 antibody (HA721741) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721741) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of HT-29 cells labeling TIMP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721741, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.