MICU2 Recombinant Rabbit Monoclonal Antibody [PSH01-84]
cat.: HA721742
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MICU2 aa 23-434. |
| Positive control: | LNCaP cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human heart tissue, human kidney tissue, human liver tissue, mouse heart tissue, rat kidney tissue, LNCaP. |
| Subcellular location: | Mitochondrion |
| Recommended Dilutions:
WB IHC-P IF-Tissue FC |
1:1,000 1:200-1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: Q8IYU8 Human | Q8CD10 Mouse | Q99P63 Rat |
| Alternative names: | 1110008L20Rik EF hand domain containing family member A1 EF hand domain family A1 EF hand domain family member A1 EF-hand domain-containing family member A1 EFHA1 EFHA1 EF hand domain family member A1 EFHA1_HUMAN FLJ25016 FLJ34588 Smhs2 homolog |
Images
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Fig1:
Western blot analysis of MICU2 on different lysates with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution. Lane 1: LNCaP cell lysate, 30 µg/Lane. Lane 2: NIH/3T3 cell lysate, 30 µg/Lane. Lane 3: PC-12 cell lysate, 30 µg/Lane. Lane 4: Human kidney tissue lysate, 30 µg/Lane. Lane 5: Human liver tissue lysate, 30 µg/Lane. Lane 6: Mouse heart tissue lysate, 30 µg/Lane. Lane 7: Mouse kidney tissue lysate, 30 µg/Lane. Lane 8: Rat kidney tissue lysate, 30 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721742) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunofluorescence analysis of paraffin-embedded human heart tissue labeling MICU2 with Rabbit anti-MICU2 antibody (HA721742) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721742, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig8:
Flow cytometric analysis of LNCaP cells labeling MICU2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721742, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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