F4/80 Recombinant Rabbit Monoclonal Antibody [PSH01-87]
cat.: HA721745
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: PSH01-87
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 102 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse F4/80 aa 1-650 / 931.
Positive control: RAW264.7(no heat) cell lysate, mouse liver tissue, mouse spleen tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue

1:1,000
1:5,000
1:500
Uniprot #: SwissProt: Q61549 Mouse
Alternative names: ADGRE1 Adhesion G protein coupled receptor E1 Adhesion G protein-coupled receptor E1 AGRE1_HUMAN Cell surface glycoprotein EMR1 Cell surface glycoprotein F4/80 DD7A5 7 Egf like module containing mucin like hormone receptor like 1 Egf like module containing mucin like hormone receptor like sequence 1 EGF like module receptor 1 EGF TM7 EGF-like module receptor 1 EGF-like module-containing mucin-like hormone receptor-like 1 EGFTM7 EMR 1 EMR1 EMR1 hormone receptor Gpf480 Ly71 Lymphocyte antigen 71 TM7LN3
Images
HA721745_1.jpg Fig1: Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721745) at 1/1,000 dilution.

Lane 1: RAW264.7(no heat) cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 102 kDa
Observed band size: 160 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721745_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA721745) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721745) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721745_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA721745) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721745) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721745_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling F4/80 with Rabbit anti-F4/80 antibody (HA721745) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721745, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721745_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling F4/80 with Rabbit anti-F4/80 antibody (HA721745) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721745, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.