eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89]
cat.: HA721747
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH01-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human eIF4A2 aa 1-407 / 407. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, PC-3M cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human brain tissue lysate, mouse testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IP |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q14240 Human | P10630 Mouse | Q5RKI1 Rat |
| Alternative names: | ATP dependent RNA helicase eIF4A 2 ATP-dependent RNA helicase eIF4A-2 BM-010 DDX2B eIF 4A II eIF-4A-II EIF4A eIF4A II eIF4A-II EIF4A2 EIF4F Eukaryotic initiation factor 4A II Eukaryotic initiation factor 4A-II eukaryotic translation initiation factor 4A isoform 2 Eukaryotic translation initiation factor 4A2 IF4A2_HUMAN N-terminally processed |
Images
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Fig1:
Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (HA721747) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-3M cell lysate Lane 4: 293T cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-12 cell lysate Lane 9: COS-1 cell lysate Lane 10: Human brain tissue lysate Lane 11: Mouse testis tissue lysate Lane 12: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721747) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.