SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-91]
cat.: HA721749
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH01-91
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SHARP2 aa 151-412 / 412.
Positive control: MCF7 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, A431 cell lysate.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O14503 Human
Alternative names: BHE40_HUMAN bHLHB2 bHLHe40 Class B Basic Helix Loop Helix Protein 2 Class B basic helix-loop-helix protein 2 Class E basic helix loop helix protein 40 Class E basic helix-loop-helix protein 40 Clast5 DEC1 Differentially expressed in chondrocytes protein 1 E47 interaction protein 1 EIP1 Enhancer of split and hairy related protein 2 Enhancer-of-split and hairy-related protein 2 SHARP 2 SHARP-2 Stimulated by retinoic acid gene 13 protein STRA13
Images
HA721749_1.jpg Fig1: Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (HA721749) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: 293T cell lysate
Lane 5: A431 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.