Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH01-91 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 46 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human SHARP2 aa 151-412 / 412. |
Positive control: | MCF7 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, A431 cell lysate. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: O14503 Human |
Alternative names: | BHE40_HUMAN bHLHB2 bHLHe40 Class B Basic Helix Loop Helix Protein 2 Class B basic helix-loop-helix protein 2 Class E basic helix loop helix protein 40 Class E basic helix-loop-helix protein 40 Clast5 DEC1 Differentially expressed in chondrocytes protein 1 E47 interaction protein 1 EIP1 Enhancer of split and hairy related protein 2 Enhancer-of-split and hairy-related protein 2 SHARP 2 SHARP-2 Stimulated by retinoic acid gene 13 protein STRA13 |
Fig1:
Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (HA721749) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |