SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-91]
cat.: HA721749
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH01-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SHARP2 aa 151-412 / 412. |
| Positive control: | MCF7 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, A431 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O14503 Human |
| Alternative names: | BHE40_HUMAN bHLHB2 bHLHe40 Class B Basic Helix Loop Helix Protein 2 Class B basic helix-loop-helix protein 2 Class E basic helix loop helix protein 40 Class E basic helix-loop-helix protein 40 Clast5 DEC1 Differentially expressed in chondrocytes protein 1 E47 interaction protein 1 EIP1 Enhancer of split and hairy related protein 2 Enhancer-of-split and hairy-related protein 2 SHARP 2 SHARP-2 Stimulated by retinoic acid gene 13 protein STRA13 |
Images
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Fig1:
Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (HA721749) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.