HA tag Recombinant Rabbit Multiclonal Antibody [PSH01-92]
cat.: HA721750
| Product Type: | Recombinant Rabbit multiclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, IP |
| Clone number: | PSH01-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein. |
| Positive control: | N Terminal fusion protein and C Terminal fusion protein. |
| Recommended Dilutions:
WB IF-Cell IP |
1:5,000 1:1,000 1-2μg/sample |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
|
Fig1:
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (HA721750) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate Lane 4: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate Lane 5: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721750) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA721750) at 1/1,000 dilution. 293T cells, transfected with HA-tagged empty control, Nanos homolog 3 (N-terminal) or CLK4 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA721750) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
HA tag was immunoprecipitated from 0.2 mg 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate with HA721750 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/20,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate (input) Lane 2: HA721750 IP in 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate Lane 3: Mouse IgG instead of HA721750 in 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 35 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.