Cre recombinase Recombinant Rabbit Monoclonal Antibody [PSH01-93]
cat.: HA721751
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IHC-P, IF-Tissue, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Cre recombinase aa 1-343 / 343. |
| Positive control: | 293T transfected with Cre recombinase cell lysate, 293T transfected with Cre recombinase cell. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IF-Cell FC |
1:1,000 1:10,000 1:20,000 1:20,000 1:1,000 |
| Uniprot #: | SwissProt: P06956 Bacteriophage P1 |
| Alternative names: | Cre Cyclization recombinase Recombinase cre |
Images
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Fig1:
Western blot analysis of Cre recombinase on different lysates with Rabbit anti-Cre recombinase antibody (HA721751) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with Cre recombinase cell lysate Lysates/proteins at 50 ng/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721751) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded 293T transfected with or without Cre recombinase cells with Rabbit anti-Cre recombinase antibody (HA721751) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721751) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded 293T transfected with or without Cre recombinase cells labeling Cre recombinase with Rabbit anti-Cre recombinase antibody (HA721751) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721751, green) at 1/20,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunocytochemistry analysis of 293T transfected with or without Cre recombinase cells labeling Cre recombinase with Rabbit anti-Cre recombinase antibody (HA721751) at 1/20,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cre recombinase antibody (HA721751) at 1/20,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of 293T transfected with or without Cre recombinase cells labeling Cre recombinase. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721751, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.