FAM134B Recombinant Rabbit Monoclonal Antibody [PSH01-94]
cat.: HA721752
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH01-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FAM134B aa 318-497 / 497. |
| Positive control: | HEK-293 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate, TT cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, Jurkat. |
| Subcellular location: | olgi apparatus, cis-Golgi network membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q9H6L5 Human | Q8VE91 Mouse | Q5FVM3 Rat |
| Alternative names: | Reticulophagy regulator 1 RETREG1 Reticulophagy receptor 1 FAM134B JK1 |
Images
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Fig1:
Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA721752) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (30 µg/Lane) Lane 2: U-2 OS cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: HeLa cell lysate (30 µg/Lane) Lane 5: HepG2 cell lysate (30 µg/Lane) Lane 6: TT cell lysate (30 µg/Lane) Lane 7: Jurkat cell lysate (30 µg/Lane) Lane 8: NIH/3T3 cell lysate (30 µg/Lane) Lane 9: C2C12 cell lysate (30 µg/Lane) Lane 10: PC-12 cell lysate (30 µg/Lane) Lane 11: C6 cell lysate (30 µg/Lane) Lane 12: Mouse liver tissue lysate (30 µg/Lane) Lane 13: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 70 kDa Exposure time: 6 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.