RNF114 Recombinant Rabbit Monoclonal Antibody [PSH01-99]
cat.: HA721757
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH01-99
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RNF114 aa 1-228 / 228.
Positive control: SK-Br-3 cell lysate, Jurkat cell lysate.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9Y508 Human
Alternative names: 1110008J21Rik AI225886 AW549494 MGC112560 OTTMUSP00000001082 OTTMUSP00000017392 PSORS12 RING finger protein 114 RN114_HUMAN RNF114 RP23-118A2.1 Zfp228 Zfp313 Zinc finger protein 228 Zinc finger protein 313 ZNF228 ZNF313
Images
HA721757_1.jpg Fig1: Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA721757) at 1/1,000 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721757) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721757_2.jpg Fig2: Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA721757) at 1/1,000 dilution.

Lane 1: 293T-si NT cell lysate
Lane 2: 293T-si RNF114 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721757) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.