RNF114 Recombinant Rabbit Monoclonal Antibody [PSH01-99]
cat.: HA721757
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH01-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RNF114 aa 1-228 / 228. |
| Positive control: | SK-Br-3 cell lysate, Jurkat cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Y508 Human |
| Alternative names: | 1110008J21Rik AI225886 AW549494 MGC112560 OTTMUSP00000001082 OTTMUSP00000017392 PSORS12 RING finger protein 114 RN114_HUMAN RNF114 RP23-118A2.1 Zfp228 Zfp313 Zinc finger protein 228 Zinc finger protein 313 ZNF228 ZNF313 |
Images
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Fig1:
Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA721757) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721757) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA721757) at 1/1,000 dilution. Lane 1: 293T-si NT cell lysate Lane 2: 293T-si RNF114 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721757) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.