Mouse LIF Recombinant Rabbit Monoclonal Antibody [PSH02-00]
cat.: HA721758
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22.3 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse LIF aa 24-203 (P09056). |
| Positive control: | Recombinant mouse Lif protein (HA210735). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-01] to Mouse LIF (Detector) (HA721759) and recombinant standard Mouse LIF (HA210735) as the standard. The reference range value is 8.2-2000 pg/ml. |
| Uniprot #: | SwissProt: P09056 Mouse |
| Alternative names: | CDF Cholinergic Differentiation Factor D factor DIA Differentiation inducing factor differentiation inhibitory activity Differentiation stimulating factor Differentiation-stimulating factor Emfilermin Hepatocyte stimulating factor III HILDA Human interleukin in DA cells Leukemia inhibitory factor LIF LIF_HUMAN Melanoma derived LPL inhibitor Melanoma-derived LPL inhibitor MLPLI |
Images
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Fig1:
Sandwich ELISA analysis of mouse Lif matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721758) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse Lif protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-01]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.