Mouse Trem2 Recombinant Rabbit Monoclonal Antibody [PSH02-02]
cat.: HA721760
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse TREM2 aa 19-171. |
| Positive control: | Recombinant mouse Trem2 protein (HA210924). |
| Subcellular location: | Cell membrane. Membrane. Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-03] to Mouse Trem2 (Detector) (HA721761). The reference range value is 37-3000 pg/ml. |
| Uniprot #: | SwissProt: Q99NH8 Mouse |
| Alternative names: | TREM 2 TREM-2 TREM2 TREM2_HUMAN TREM2a TREM2b TREM2c Trggering receptor expressed on myeloid cells 2 Trggering receptor expressed on myeloid cells 2a Triggering receptor expressed on monocytes 2 Triggering receptor expressed on myeloid cells 2 |
Images
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Fig1:
Sandwich ELISA analysis of mouse Trem2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721760) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse Trem2 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody [PSH02-03]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.