Transcription factor AP-2-alpha Recombinant Rabbit Monoclonal Antibody [PSH02-05]
cat.: HA721764
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH02-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TFAP2A aa 1-437 / 437. |
| Positive control: | JAR cell lysate, HepG2 cell lysate, SiHa cell lysate, HepG2, human breast tissue, mouse eye tissue, rat breast tissue, rat skin tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200-1:1,000 1:100 |
| Uniprot #: | SwissProt: P05549 Human | P34056 Mouse | P58197 Rat |
| Alternative names: | Activating enhancer binding protein 2 alpha Activating enhancer-binding protein 2-alpha Activator protein 2 AP 2 transcription factor AP 2alpha AP-2 AP-2 transcription factor AP2 AP2 Transcription Factor AP2-alpha AP2A_HUMAN AP2TF BOFS FLJ51761 TFAP 2 TFAP 2A TFAP2 TFAP2A Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) Transcription factor AP-2-alpha Transcription factor AP2 alpha |
Images
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Fig1:
Western blot analysis of Transcription factor AP-2-alpha on different lysates with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721764) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling Transcription factor AP-2-alpha with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721764) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse eye tissue with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721764) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721764) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721764) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721764) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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