MYL9 Recombinant Rabbit Monoclonal Antibody [PSH02-07]
cat.: HA721766
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MYL9 aa 1-172 / 172. |
| Positive control: | Jurkat cell lysate, MDA-MB-231 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, A431. |
| Subcellular location: | Cytoplasm, cytoskeleton, cell cortex. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P24844 Human | Q9CQ19 Mouse | Q64122 Rat |
| Alternative names: | 20 kDa myosin light chain Human 20kDa myosin light chain (MLC2) mRNA complete cds LC20 MGC3505 MLC 2 MLC-2C MLC2 MLY 9 MRLC1 MYL9 MYL9_HUMAN Myosin light chain 9 regulatory Myosin light polypeptide 9 regulatory myosin regulatory light chain 1 Myosin regulatory light chain 2 Myosin regulatory light chain 2 smooth muscle isoform Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light polypeptide 9 Myosin RLC Myosin vascular smooth muscle light chain 2 MYRL2 OTTHUMP00000030857 smooth muscle isoform |
Images
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Fig1:
Western blot analysis of MYL9 on different lysates with Rabbit anti-MYL9 antibody (HA721766) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Predicted band size: 20 kDa Observed band size: 18 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721766) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of A431 cells labeling MYL9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721766, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.