Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-08]
cat.: HA721767
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH02-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-18 aa 37-193. |
| Positive control: | U-2 OS cell lysate, A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, human liver tissue, human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:1,000 1:5,000-1:10,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q14116 Human |
| Alternative names: | Iboctadekin IFN gamma inducing factor IFN-gamma-inducing factor IGIF IL 1 gamma IL 18 IL 1g IL-1 gamma IL-18 IL1 gamma IL18 IL18 protein IL18_HUMAN IL1F4 IL1g IL1gamma ILIF4 Interferon gamma inducing factor Interferon gamma-inducing factor Interleukin 1 gamma Interleukin 18 (interferon-gamma-inducing factor) Interleukin 18 Interleukin-1 gamma Interleukin-18 Interleukin18 MGC12320 |
Images
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Fig1:
Western blot analysis of Human IL-18 on different lysates with Rabbit anti-Human IL-18 antibody (HA721767) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721767) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Human IL-18 antibody (HA721767) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721767) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Human IL-18 antibody (HA721767) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721767) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Human IL-18 antibody (HA721767) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721767) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
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Fig6:
Human IL-18 was immunoprecipitated from 0.2 mg A549 cell lysate with HA721767 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721767 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: Rabbit IgG instead of HA721767 in A549 cell lysate Lane 3: HA721767 IP in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.