cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH02-10]
cat.: HA721769
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human cIAP1 aa 201-618 / 618. |
| Positive control: | HT-29 cell lysate, TF-1 cell lysate, HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, Jurkat cell lysate, A549 cell lysate, HEK-293 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q13490 Human |
| Alternative names: | API 1 API1 Apoptosis inhibitor 1 Baculoviral IAP repeat containing 2 Baculoviral IAP repeat containing protein 2 Baculoviral IAP repeat-containing protein 2 BIRC 2 BIRC2 BIRC2_HUMAN C IAP1 C-IAP1 Cellular inhibitor of apoptosis 1 cellular inhibitor of apoptosis protein 1 cIAP 1 cIAP1 HIAP 2 HIAP-2 HIAP2 IAP 2 IAP homolog B IAP-2 IAP2 Inhibitor of apoptosis protein 2 MIHB NFR2 TRAF signalling complex protein RING finger protein 48 RNF 48 RNF48 TNFR2 TRAF signaling complex protein 2 TNFR2-TRAF-signaling complex protein 2 |
Images
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Fig1:
Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA721769) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: TF-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: HeLa cell lysate Lane 5: THP-1 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721769) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA721769) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-cIAP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 78 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721769) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.