KMT2C/ MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11]
cat.: HA721770
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 541 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KMT2C aa 3,251-3,550 / 4,911. |
| Positive control: | U-2 OS cell lysate, MCF7 cell lysate, HCT 116 cell lysate, rat skeletal muscle tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8NEZ4 Human | Q8BRH4 Mouse Entrez Gene: 502710 Rat |
| Alternative names: | Histone-lysine N-methyltransferase 2C EC:2.1.1.364 Lysine N-methyltransferase 2C Homologous to ALR protein Myeloid/lymphoid or mixed-lineage leukemia protein 3 KMT2C HALR KIAA1506 MLL3 |
Images
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Fig1:
Western blot analysis of KMT2C/ MLL3 on different lysates with Rabbit anti-KMT2C/ MLL3 antibody (HA721770) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 541 kDa Observed band size: 541 kDa Exposure time: 1 minute 36 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721770) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.