TRIB3 Recombinant Rabbit Monoclonal Antibody [PSH02-13]
cat.: HA721772
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRIB3 aa 1-358 / 358. |
| Positive control: | HepG2 cell lysate, HCT 116 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, K-562 cell lysate, 293T cell lysate, HT-29 cell lysate, HUVEC cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q96RU7 Human |
| Alternative names: | C20orf97 Neuronal cell death inducible putative kinase Neuronal cell death-inducible putative kinase NIPK p65 interacting inhibitor of NF-kappaB p65-interacting inhibitor of NF-kappa-B SINK SKIP 3 SKIP3 TRB 3 TRB-3 TRB3 TRIB 3 Trib3 TRIB3_HUMAN Tribbles homolog 3 Tribbles pseudokinase 3 Tribbles3 |
Images
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Fig1:
Western blot analysis of TRIB3 on different lysates with Rabbit anti-TRIB3 antibody (HA721772) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HCT 116 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: HT-29 cell lysate Lane 8: HUVEC cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 40 kDa Observed band size: 42 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721772) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.