TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]
cat.: HA721776
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB, FC
Clonality: Monoclonal
Clone number: PSH02-17
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 81 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TRIM56 aa 350-755 (Q9BRZ2).
Positive control: HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, 293T cell lysate, A375 cell lysate, COS-1 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse lung tissue lysate, HeLa, NIH/3T3, C2C12, PC-12.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  FC

1:1,000
1:1,000
Uniprot #: SwissProt: Q9BRZ2 Human | Q80VI1 Mouse | A0A8I6G8D1 Rat
Alternative names: A130009K11Rik DKFZp667O116 E3 ubiquitin-protein ligase TRIM56 FLJ35608 Gm452 MGC37358 OTTMUSP00000027392 RING finger protein 109 RNF109 TRI56_HUMAN Trim56 Tripartite motif containing protein 56 Tripartite motif-containing protein 56
Images
HA721776_1.jpg Fig1: Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: A549 cell lysate
Lane 4: 293T cell lysate
Lane 5: A375 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: C2C12 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse lung tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 81 kDa
Observed band size: 81 kDa

Exposure time: 3 minutes 54 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721776_2.jpg Fig2: Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate (20 µg/Lane)
Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane)

Predicted band size: 81 kDa
Observed band size: 81 kDa

Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721776_3.jpg Fig3: Flow cytometric analysis of HeLa cells labeling TRIM56.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721776_4.jpg Fig4: Flow cytometric analysis of NIH/3T3 cells labeling TRIM56.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721776_5.jpg Fig5: Flow cytometric analysis of C2C12 cells labeling TRIM56.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721776_6.jpg Fig6: Flow cytometric analysis of PC-12 cells labeling TRIM56.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.