TRIM56 Recombinant Rabbit Monoclonal Antibody [PSH02-17]
cat.: HA721776
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRIM56 aa 350-755 (Q9BRZ2). |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, 293T cell lysate, A375 cell lysate, COS-1 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse lung tissue lysate, HeLa, NIH/3T3, C2C12, PC-12. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9BRZ2 Human | Q80VI1 Mouse | A0A8I6G8D1 Rat |
| Alternative names: | A130009K11Rik DKFZp667O116 E3 ubiquitin-protein ligase TRIM56 FLJ35608 Gm452 MGC37358 OTTMUSP00000027392 RING finger protein 109 RNF109 TRI56_HUMAN Trim56 Tripartite motif containing protein 56 Tripartite motif-containing protein 56 |
Images
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Fig1:
Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: 293T cell lysate Lane 5: A375 cell lysate Lane 6: COS-1 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-12 cell lysate Lane 9: Mouse lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA721776) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate (20 µg/Lane) Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Flow cytometric analysis of NIH/3T3 cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of C2C12 cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Flow cytometric analysis of PC-12 cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721776, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.