ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]
cat.: HA721777
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH02-18
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ZIP Kinase aa 250-454 (O43293).
Positive control: A431 cell lysate, A549 cell lysate, 293T cell lysate, SiHa cell lysate, HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, Caco-2 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, human kidney tissue lysate.
Subcellular location: Nucleus, Cytoplasm
Recommended Dilutions:
  WB

1:1,000-1:2,000
Uniprot #: SwissProt: O43293 Human
Alternative names: DAP kinase 3 DAP like kinase DAP-like kinase Dapk 3 DAPK3 DAPK3_HUMAN Death associated kinase 3 Death associated protein kinase 3 Death-associated protein kinase 3 Dlk EC 2.7.11.1 FLJ36473 MYPT1 kinase ZIP ZIP kinase ZIP kinase isoform ZIP-kinase ZIPK zipper-interacting protein kinase
Images
HA721777_1.jpg Fig1: Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA721777) at 1/1,000 dilution.

Lane 1: A431 cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: SiHa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Hela cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Caco-2 cell lysate
Lane 9: K-562 cell lysate
Lane 10: MCF7 cell lysate
Lane 11: U-2 OS cell lysate
Lane 12: Human kidney tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 53 kDa
Observed band size: 50 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721777_2.jpg Fig2: Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA721777) at 1/2,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si ZIP Kinase cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721777) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.