ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]
cat.: HA721777
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ZIP Kinase aa 250-454 (O43293). |
| Positive control: | A431 cell lysate, A549 cell lysate, 293T cell lysate, SiHa cell lysate, HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, Caco-2 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, human kidney tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: O43293 Human |
| Alternative names: | DAP kinase 3 DAP like kinase DAP-like kinase Dapk 3 DAPK3 DAPK3_HUMAN Death associated kinase 3 Death associated protein kinase 3 Death-associated protein kinase 3 Dlk EC 2.7.11.1 FLJ36473 MYPT1 kinase ZIP ZIP kinase ZIP kinase isoform ZIP-kinase ZIPK zipper-interacting protein kinase |
Images
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Fig1:
Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA721777) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: HepG2 cell lysate Lane 6: Hela cell lysate Lane 7: Jurkat cell lysate Lane 8: Caco-2 cell lysate Lane 9: K-562 cell lysate Lane 10: MCF7 cell lysate Lane 11: U-2 OS cell lysate Lane 12: Human kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 53 kDa Observed band size: 50 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA721777) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si ZIP Kinase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721777) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.