Human HGF Recombinant Rabbit Monoclonal Antibody [PSH02-06]
cat.: HA721778
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HGF aa 32-494 (P14210). |
| Positive control: | Recombinant Human HGF protein. |
| Subcellular location: | extracellular region, extracellular space |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-07] to Human HGF (Detector) (HA721779). The reference range value is 12.35-3000pg/ml. |
| Uniprot #: | SwissProt: P14210 Human |
| Alternative names: | DFNB39 F TCF Fibroblast derived tumor cytotoxic factor Hepatocyte growth factor (hepapoietin A; scatter factor) Hepatocyte growth factor Hepatocyte growth factor beta chain Hepatocyte growth factor precursor Hepatopoietin A Hepatopoietin-A Hgf HGF_HUMAN HGFB HPTA Lung fibroblast derived mitogen OTTHUMP00000161349 OTTHUMP00000206710 OTTHUMP00000206711 OTTHUMP00000206712 OTTHUMP00000206713 OTTHUMP00000206730 Scatter factor SF |
Images
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Fig1:
Sandwich ELISA analysis of human HGF matched pair antibodies. Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721778) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human HGF protein starting from 3000 pg/ml to 0 pg/ml and detect antibody [PSH02-07]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.