Human FGF-2 Recombinant Rabbit Monoclonal Antibody [PSH02-09]
cat.: HA721781
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FGF-2 aa 143-288 (P09038). |
| Positive control: | Recombinant Human FGF-2 protein (HA210834). |
| Subcellular location: | Secreted, Nucleus. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-08] to Human FGF-2 (Detector) (HA721780). The reference range value is 2.74-2000pg/ml. |
| Uniprot #: | SwissProt: P09038 Human |
| Alternative names: | Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth factor 2 (basic) Fibroblast growth factor 2 Fibroblast growth factor, basic HBGF 2 HBGF-2 HBGF2 HBGH 2 HBGH2 Heparin binding growth factor 2 precursor Heparin-binding growth factor 2 Prostatropin |
Images
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Fig1:
Sandwich ELISA analysis of Human FGF-2 matched pair antibodies. Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721780) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human FGF-2 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-09]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.