Human EGF Recombinant Rabbit Monoclonal Antibody [PSH02-19]
cat.: HA721782
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 134 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EGF aa 971-1023 (P01133). |
| Positive control: | Recombinant Human EGF protein. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-20] to Human EGF (Detector) (HA721783). The reference range value is 1.23-300pg/ml. |
| Uniprot #: | SwissProt: P01133 Human |
| Alternative names: | Beta urogastrone beta-urogastrone EGF EGF_HUMAN Epidermal growth factor HOMG4 OTTHUMP00000219721 OTTHUMP00000219722 Pro epidermal growth factor URG Urogastrone |
Images
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Fig1:
Sandwich ELISA analysis of Human EGF matched pair antibodies. Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721782) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human EGF protein starting from 300 pg/ml to 0 pg/ml and detect antibody [PSH02-20]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.