Rat IL-10 Recombinant Rabbit Monoclonal Antibody [PSH02-22]
cat.: HA721787
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat IL-10 aa 19-178 (P29456). |
| Positive control: | Recombinant Rat IL-10 protein (HA210714). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-21] to Rat IL-10 (Capture) (HA721786). The reference range value is 13.7-10000pg/ml. |
| Uniprot #: | SwissProt: P29456 Rat |
| Alternative names: | CSIF Cytokine synthesis inhibitory factor GVHDS IL 10 IL-10 IL10 IL10_HUMAN IL10A Interleukin 10 Interleukin-10 MGC126450 MGC126451 T-cell growth inhibitory factor TGIF |
Images
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Fig1:
Sandwich ELISA analysis of Rat IL-10 matched pair antibodies. Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721786) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-10 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody [PSH02-22]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.