Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | IHC-P, WB |
Clonality: | Monoclonal |
Clone number: | PSH02-82 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 4 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human beta Amyloid 1-40 peptide. |
Positive control: | APP/PS1, 6-month mouse of AD brain tissue. |
Subcellular location: | Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle. |
Recommended Dilutions:
IHC-P WB |
1:1,000 1:1,000 |
Uniprot #: | SwissProt: P05067 Human | P12023 Mouse |
Alternative names: | A4 A4_HUMAN AAA ABETA ABPP AICD-50 AICD-57 AICD-59 AID(50) AID(57) AID(59) Alzheimer disease amyloid protein Amyloid intracellular domain 50 Amyloid intracellular domain 57 Amyloid intracellular domain 59 APP APPI Beta amyloid protein 42 Beta APP42 Beta-APP40 Beta-APP42 C31 Cerebral vascular amyloid peptide CVAP Gamma-CTF(50) Gamma-CTF(57) Gamma-CTF(59) PN-II PreA4 Protease nexin-II S-APP-alpha S-APP-beta |
Fig1:
Western blot analysis of beta Amyloid 1-40 on different peptides with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution. Lane 1: Human Aβ37 full length peptide (negative) Lane 2: Human Aβ38 full length peptide (negative) Lane 3: Human Aβ39 full length peptide (negative) Lane 4: Human Aβ40 full length peptide (positive) Lane 5: Human Aβ41 full length peptide (negative) Lane 6: Human Aβ42 full length peptide (negative) Lane 7: Human Aβ43 full length peptide (negative) Lane 8: Mouse Aβ40 full length peptide (positive) Lysates/proteins at 100 ng/Lane. Predicted band size: 4 kDa Observed band size: 4 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721788) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721788) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721788) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |