Phospho-PRAS40 (T246) Recombinant Rabbit Monoclonal Antibody [JE53-94]
cat.: HA721790
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE53-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr246 of human PRAS40. |
| Positive control: | HeLa cell lysate, HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q96B36 Human |
| Alternative names: | 40 kDa proline rich AKT substrate 40 kDa proline-rich AKT substrate AKT1 S1 AKT1 substrate 1 (proline rich) AKT1 substrate 1 AKT1S 1 AKT1S1 AKTS1_HUMAN Lobe MGC2865 PRAS 40 PRAS PRAS40 Proline rich akt substrate Proline rich Akt substrate 40 kDa Proline-rich AKT1 substrate 1 |
Images
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Fig1:
Western blot analysis of Phospho-PRAS40 (T246) on different lysates with Rabbit anti-Phospho-PRAS40 (T246) antibody (HA721790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with λpp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.