Phospho-MDM2 (S166) Recombinant Rabbit Monoclonal Antibody [JE64-20]
cat.: HA721791
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2. |
| Positive control: | A549 cell lysate. |
| Subcellular location: | Nucleus, nucleoplasm, nucleolus, cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q00987 Human | P23804 Mouse |
| Alternative names: | ACTFS Double minute 2 protein E3 ubiquitin-protein ligase Mdm2 Hdm 2 Hdm2 HDMX MDM 2 MDM2 MDM2 oncogene E3 ubiquitin protein ligase Mdm2 p53 E3 ubiquitin protein ligase homolog Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa MDM2_HUMAN MDM2BP Mouse Double Minute 2 MTBP Murine Double Minute Chromosome 2 Oncoprotein Mdm2 p53 Binding Protein Mdm2 p53-binding protein Mdm2 Ubiquitin protein ligase E3 Mdm2 Ubiquitin protein ligase E3 Mdm2 |
Images
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Fig1:
Western blot analysis of Phospho-MDM2 (S166) on different lysates with Rabbit anti-Phospho-MDM2 (S166) antibody (HA721791) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with λpp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721791) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.