Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81]
cat.: HA721792
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE43-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 201 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues around Thr1462 of human tuberin. |
| Positive control: | SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate. |
| Subcellular location: | Cytoplasm, Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P49815 Human | Q61037 Mouse | P49816 Rat |
| Alternative names: | FLJ43106 LAM OTTHUMP00000158940 OTTHUMP00000198394 OTTHUMP00000198395 PPP1R160 Protein phosphatase 1, regulatory subunit 160 TSC complex subunit 2 tsc2 TSC2_HUMAN TSC4 TSC4 gene, formerly TSC4, formerly Tuberin Tuberous sclerosis 2 Tuberous sclerosis 2 protein Tuberous sclerosis 2 protein homolog |
Images
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Fig1:
Western blot analysis of Phospho-Tuberin (T1462) on different lysates with Rabbit anti-Phospho-Tuberin (T1462) antibody (HA721792) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour, then treated with λpp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721792) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.