Phospho-RSK1 p90 (T573) Recombinant Rabbit Monoclonal Antibody [JE50-12]
cat.: HA721793
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE50-12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr573 of human RSK1 p90.
Positive control: K-562 treated with 500nM PMA for 15 minutes cell lysate, HeLa treated with 200nM PMA for 4 hours cell lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q15418 Human | P18653 Mouse | Q63531 Rat
Alternative names: 90 kDa ribosomal protein S6 kinase 1 dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 dJ590P13.1 EC 2.7.11.1 HU 1 HU1 KS6A1_HUMAN MAP kinase activated protein kinase 1a MAP kinase-activated protein kinase 1a MAPK-activated protein kinase 1a MAPKAP kinase 1a MAPKAPK-1a MAPKAPK1A MGC79981 Mitogen-activated protein kinase-activated protein kinase 1A OTTHUMP00000004113 p90 RSK1 p90-RSK 1 p90rsk p90RSK1 p90S6K pp90RSK1 Ribosomal protein S6 kinase 90kD 1 Ribosomal protein S6 kinase 90kD polypeptide 1 Ribosomal protein S6 kinase 90kDa polypeptide 1 Ribosomal protein S6 kinase alpha 1 Ribosomal protein S6 kinase alpha-1 Ribosomal protein S6 kinase polypeptide 1 Ribosomal S6 kinase 1 RPS6K1 alpha rps6ka Rps6ka1 RSK 1 RSK 1 p90 RSK RSK-1 RSK1 S6K alpha 1 S6K-alpha-1
Images
HA721793_1.jpg Fig1: Western blot analysis of Phospho-RSK1 p90 (T573) on different lysates with Rabbit anti-Phospho-RSK1 p90 (T573) antibody (HA721793) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: K-562 treated with 500nM PMA for 15 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 200nM PMA for 4 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721793) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.