Phospho-CaMKII (T286) Recombinant Rabbit Monoclonal Antibody [JE51-81]
cat.: HA721794
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE51-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr286 of human CaMKII. |
| Positive control: | Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Synapse, Postsynaptic density, Cell projection, dendritic spine, dendrite. |
| Recommended Dilutions:
WB |
1,000 |
| Uniprot #: | SwissProt: Q13554 Human | Q13555 Human | Q13557 Human | Q9UQM7 Human |
| Alternative names: | Calcium/calmodulin dependent protein kinase II alpha Calcium/calmodulin dependent protein kinase II beta Calcium/calmodulin dependent protein kinase II delta Calcium/calmodulin dependent protein kinase II gamma Calcium/calmodulin-dependent protein kinase type II subunit alpha CaM kinase II alpha CaM kinase II CaM kinase II beta CaM kinase II delta CaM kinase II gamma CaM kinase II subunit alpha CaMK-II subunit alpha CAMK2 Camk2a CAMK2B CAMK2D CAMK2G CAMKA KCC2A_HUMAN |
Images
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Fig1:
Western blot analysis of Phospho-CaMKII (T286) on different lysates with Rabbit anti-Phospho-CaMKII (T286) antibody (HA721794) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 54~73 kDa Observed band size: 50~60 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721794) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.