Phospho-IKB alpha (S36) Recombinant Rabbit Monoclonal Antibody [JE56-65]
cat.: HA721802
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser36 of human IKB alpha. |
| Positive control: | C6 treated with 100n/mL Calyculin A for 1 hour cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Z1E3 Mouse | Q63746 Rat |
| Alternative names: | I kappa B alpha I-kappa-B-alpha IkappaBalpha IkB-alpha IKBA IKBA_HUMAN IKBalpha MAD 3 MAD3 Major histocompatibility complex enhancer-binding protein MAD3 NF kappa B inhibitor alpha NF-kappa-B inhibitor alpha NFKBI NFKBIA Nuclear factor of kappa light chain gene enhancer in B cells Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha |
Images
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Fig1:
Western blot analysis of Phospho-IKB alpha (S36) on different lysates with Rabbit anti-Phospho-IKB alpha (S36) antibody (HA721802) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721802) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.