SCP3 Recombinant Rabbit Monoclonal Antibody [PSH02-24]
cat.: HA721804
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH02-24
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SCP3 aa 35-236 / 236 (Q8IZU3).
Positive control: Human testis tissue.
Subcellular location: Nucleus, Chromosome, centromere.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:1,000
Uniprot #: SwissProt: Q8IZU3 Human
Alternative names: choline phosphotransferase 1 chpt1 COR 1 COR1 MGC71888 RNASCP3 SCP 3 SCP-3 SCP3 SPGF4 Sycp 3 Sycp3 SYCP3_HUMAN Synaptonemal complex protein 3
Images
HA721804_1.jpg Fig1: Western blot analysis of SCP3 on SCP3 recombinant protein with Rabbit anti-SCP3 antibody (HA721804) at 1/1,000 dilution.

Lysates/proteins at 30 ng/Lane.

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721804) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721804_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SCP3 antibody (HA721804) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721804) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.