HA721806

TXNRD2 Recombinant Rabbit Monoclonal Antibody [PSH02-26]

cat.: HA721806

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH02-26

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 57 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human TXNRD2 aa 1-524 / 524.
Positive control:	HEK-293 cell lysate, HeLa cell lysate, A431 cell lysate, K-562 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human prostate tissue, human liver tissue, mouse liver tissue, rat liver tissue.
Subcellular location:	Mitochondrion.
Recommended Dilutions: WB IHC-P IF-Tissue	1:1,000 1:200-1:1,000 1:50
Uniprot #:	SwissProt: Q9NNW7 Human \| Q9JLT4 Mouse \| Q9Z0J5 Rat
Alternative names:	mitochondrial Selenoprotein Z SelZ Thioredoxin reductase 2 Thioredoxin reductase 2 mitochondrial Thioredoxin reductase 3 Thioredoxin reductase beta Thioredoxin reductase TR3 TR 3 TR TR beta TR-beta TR3 TRXR 2 TRXR2 TRXR2_HUMAN TXNRD 2 Txnrd2

Images

Fig1: Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (HA721806) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat heart tissue lysate (40 µg/Lane)
Lane 7: Mouse liver tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Lane 9: Human kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse kidney tissue lysate (40 µg/Lane)
Lane 11: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 57 kDa
Observed band size: 57 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721806) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TXNRD2 antibody (HA721806) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721806) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TXNRD2 antibody (HA721806) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721806) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-TXNRD2 antibody (HA721806) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721806) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-TXNRD2 antibody (HA721806) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721806) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunofluorescence analysis of paraffin-embedded rat liver tissue labeling TXNRD2 with Rabbit anti-TXNRD2 antibody (HA721806) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721806, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Fig7: Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (HA721806) at 1/1,000 dilution.

Lane 1: MCF7-si NT cell lysate
Lane 2: MCF7-si TXNRD2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 57 kDa
Observed band size: 57 kDa

Exposure time: 16 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721806) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.