REIIBP/NSD2 Recombinant Rabbit Monoclonal Antibody [PSH02-39]
cat.: HA721816
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH02-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NSD2 385-584 / 584 (O96028-4). |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Rat testis tissue. |
| Subcellular location: | Cytoplasm, nucleus, nucleolus |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: O96028 Hmuan | Q8BVE8 Mouse | D4A9J4 Rat |
| Alternative names: | FLJ23286 IL5 promoter REII region binding protein KIAA1090 MGC176638 MMSET MMSET type II Multiple myeloma SET domain containing protein type III Multiple myeloma SET domain protein Multiple myeloma SET domain-containing protein NSD 2 NSD2 NSD2_HUMAN Nuclear receptor binding SET domain protein 2 Nuclear SET domain-containing protein 2 Probable histone-lysine N-methyltransferase NSD2 Protein trithorax-5 REIIBP Trithorax/ash1 related protein 5 TRX5 TRX5 protein WHS Whsc1 Wolf Hirschhorn syndrome candidate 1 Wolf Hirschhorn syndrome candidate 1 protein Wolf-Hirschhorn syndrome candidate 1 protein |
Images
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Fig1:
Western blot analysis of REIIBP/NSD2 on different lysates with Rabbit anti-REIIBP/NSD2 antibody (HA721816) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded Rat testis tissue with Rabbit anti-REIIBP/NSD2 antibody (HA721816) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721816) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.