MTH1 Recombinant Rabbit Monoclonal Antibody [PSH02-40]
cat.: HA721817
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MTH1 aa 1-156 / 156 (P36639). |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, SW480 cell lysate, THP-1 cell lysate, SK-OV-3 cell lysate, U-2 OS cell lysate, human breast cancer tissue, NIH/3T3 cell lysate, C2C12 cell lysate, bEnd.3 cell lysate, RAW264.7 cell lysate, F9 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, PC-12 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, human stomach cancer tissue, human thymus tissue, rat thymus tissue, Jurkat. |
| Subcellular location: | Cytoplasm, cytosol,Mitochondrion matrix,Nucleus,Mitochondrion matrix |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200~1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P36639 Human | P53368 Mouse | P53369 Rat |
| Alternative names: | 2-hydroxy-dATP diphosphatase 7 8 dihydro 8 oxoguanine triphosphatase 7 8 oxo 7 8 dihydrodeoxyguanosine triphosphatase 8 oxo 7 8 dihydroguanosine triphosphatase 8 oxo dGTPase 8-dihydro-8-oxoguanine triphosphatase 8-oxo-dGTPase 8ODP_HUMAN MTH 1 MTH1 MutT human homolog 1 Nucleoside diphosphate linked moiety X motif 1 Nucleoside diphosphate linked moiety X type motif 1 Nucleoside diphosphate-linked moiety X motif 1 Nudix (nucleoside diphosphate linked moiety X) type motif 1 Nudix hydrolase 1 Nudix motif 1 Nudix type motif 1 NUDT 1 Nudt1 |
Images
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Fig1:
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lane 7: MCF7 cell lysate Lane 8: SW480 cell lysate Lane 9: THP-1 cell lysate Lane 10: SK-OV-3 cell lysate Lane 11: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lane 4: bEnd.3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: F9 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Mouse thymus tissue lysate Lane 12: Rat thymus tissue lysate Lane 13: Mouse testis tissue lysate Lane 14: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. Lane 1: HEK-293-si NT cell lysate Lane 2: HEK-293-si MTH1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 1 minute 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig8:
Flow cytometric analysis of Jurkat cells labeling MTH1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721817,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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