Galectin 9 Recombinant Rabbit Monoclonal Antibody [PSH02-44]
cat.: HA721821
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Galectin 9 aa 51-100 / 355. |
| Positive control: | THP-1 cell lysate, PANC-1 cell lysate, HL-60 cell lysate, A549 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, THP-1. |
| Subcellular location: | Cytoplasm, Nucleus, Secreted. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O00182 Human | O08573 Mouse |
| Alternative names: | 36 kDa beta-galactoside-binding lectin Ecalectin Gal-9 galectin 9 Galectin-9 galectin9 HOM HD 21 HOMHD21 HUAT Lectin galactoside binding soluble 9 LEG9_HUMAN LGAL S9 LGALS 9 Lgals9 LGALS9A MGC117375 MGC125973 MGC125974 Tumor antigen HOM-HD-21 UAT Urate transporter/channel Urate transporter/channel protein |
Images
|
Fig1:
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721821) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: PC-3 cell lysate (low expression) Lane 3: PANC-1 cell lysate (low expression) Lane 4: HL-60 cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 40 kDa Observed band size: 36 kDa Exposure time: 2 minutes 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of THP-1 cells labeling Galectin 9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721821, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.