Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH02-45 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 40 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human Galectin 9 aa 51-100 / 355. |
Positive control: | THP-1 cell lysate, A549 cell lysate, rat liver tissue lysate. |
Subcellular location: | Cytoplasm, Nucleus, Secreted. |
Recommended Dilutions:
WB |
1:2,000 |
Uniprot #: | SwissProt: O00182 Human | P97840 Rat |
Alternative names: | 36 kDa beta-galactoside-binding lectin Ecalectin Gal-9 galectin 9 Galectin-9 galectin9 HOM HD 21 HOMHD21 HUAT Lectin galactoside binding soluble 9 LEG9_HUMAN LGAL S9 LGALS 9 Lgals9 LGALS9A MGC117375 MGC125973 MGC125974 Tumor antigen HOM-HD-21 UAT Urate transporter/channel Urate transporter/channel protein |
Fig1:
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721822) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 35 kDa Exposure time: 2 minutes 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721822) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |