GSTA4 Recombinant Rabbit Monoclonal Antibody [JE61-65]
cat.: HA721825
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE61-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GSTA4 aa 1-100. |
| Positive control: | HEK-293 cell lysate, JAR cell lysate, human liver tissue lysate, human brain tissue lysate, human kidney tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: O15217 Human |
| Alternative names: | DKFZp686D21185 Glutathione S alkyltransferase A4 Glutathione S aralkyltransferase A4 Glutathione S aryltransferase A4 Glutathione S transferase A4 4 Glutathione S transferase A4 Glutathione S transferase alpha 4 Glutathione S-transferase A4 Glutathione S-transferase A4-4 Glutathione transferase A4 4 GST class alpha member 4 GST class-alpha member 4 GSTA4 4 GSTA4 GSTA4_HUMAN GTA4 OTTHUMP00000016624 OTTHUMP00000016625 S (hydroxyalkyl)glutathione lyase A4 |
Images
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Fig1:
Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (HA721825) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (40 µg/Lane) Lane 2: JAR cell lysate (40 µg/Lane) Lane 3: Human liver tissue lysate (40 µg/Lane) Lane 4: Human brain tissue lysate (30 µg/Lane) Lane 5: Human kidney tissue lysate (30 µg/Lane) Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721825) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (HA721825) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GSTA4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721825) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.