Merlin Recombinant Rabbit Monoclonal Antibody [PSH02-52]
cat.: HA721828
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: PSH02-52
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Merlin aa 1-350 / 595 (P35240).
Positive control: PC-3M cell lysate, HeLa cell lysate, MDA-MB-468 cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, HEK-293 cell lysate, Jurkat cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HUVEC, NIH/3T3.
Subcellular location: Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:2,000
1:100
1:1,000
Uniprot #: SwissProt: P35240 Human | P46662 Mouse | Q63648 Rat
Alternative names: ACN BANF Bilateral acoustic neuroma MERL_HUMAN Merlin Moesin ezrin radixin like protein Moesin ezrin radizin like Moesin-ezrin-radixin-like protein Neurofibromatosis 2 Neurofibromatosis type 2 Neurofibromatosis2 Neurofibromin 2 Neurofibromin-2 Neurofibromin2 NF 2 Nf2 SCH Schwannomerlin Schwannomin
Images
HA721828_1.jpg Fig1: Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721828) at 1/2,000 dilution.

Lane 1: PC-3M cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: Jurkat cell lysate (20 µg/Lane)
Lane 8: MDA-MB-231 cell lysate (negative) (20 µg/Lane)
Lane 9: COS-1 cell lysate (20 µg/Lane)
Lane 10: Neuro-2a cell lysate (20 µg/Lane)
Lane 11: NIH/3T3 cell lysate (20 µg/Lane)
Lane 12: C6 cell lysate (20 µg/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 1 minute 41 seconds; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721828) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721828_2.jpg Fig2: Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721828) at 1/2,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Merlin cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721828) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721828_3.jpg Fig3: Immunocytochemistry analysis of HUVEC cells labeling Merlin with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721828_4.jpg Fig4: Immunocytochemistry analysis of NIH/3T3 cells labeling Merlin with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721828_5.jpg Fig5: Flow cytometric analysis of NIH/3T3 cells labeling Merlin.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721828, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.