ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53]
cat.: HA721829
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATG4B aa 1-393 / 393 (Q9Y4P1). |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, MCF7 cell lysate, K-562 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Mouse kidney tissue lysate, Mouse colon tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9Y4P1 Human | Q8BGE6 Mouse | Q62625 Rat |
| Alternative names: | APG4 autophagy 4 homolog B APG4B ATG4 autophagy related 4 homolog B (S. cerevisiae) ATG4B ATG4B_HUMAN AUT like 1 cysteine endopeptidase AUT-like 1 cysteine endopeptidase AUTL1 Autophagin 1 Autophagin-1 Autophagy related 4B cysteine peptidase Autophagy related cysteine endopeptidase 1 Autophagy related protein 4 homolog B Autophagy-related cysteine endopeptidase 1 Autophagy-related protein 4 homolog B Cysteine protease ATG4B hAPG4B MGC1353 |
Images
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Fig1:
Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA721829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse brain tissue lysate Lane 11: Rat brain tissue lysate Lane 12: Mouse kidney tissue lysate Lane 13: Mouse colon tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 47 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA721829) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: COS-1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 44 kDa Observed band size: 47 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721829) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA721829) at 1/1,000 dilution. Lane 1: 293T-si NT cell lysate Lane 2: 293T-si ATG4B cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 47 kDa Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721829) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.