MLD Recombinant Rabbit Monoclonal Antibody [PSH02-54]
cat.: HA721830
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH02-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MLD aa 274-323 / 323 (O15121). |
| Positive control: | A549 cell lysate, 293T cell lysate, HeLa cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, human skin tissue, human breast carcinoma tissue, rat skin tissue. |
| Subcellular location: | Mitochondrion membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:200-1:1,000 1:200 |
| Uniprot #: | SwissProt: O15121 Human | O09005 Mouse | Q5XIF5 Rat |
| Alternative names: | Degenerative spermatocyte homolog 1 Degenerative spermatocyte homolog 1 lipid desaturase Degenerative spermatocyte homolog lipid desaturase DEGS 1 DEGS Delta 4 desaturase sphingolipid 1 Des 1 DES1 Dihydroceramide desaturase FADS7 Membrane fatty acid (lipid) desaturase Membrane lipid desaturase MGC5079 MIG15 Migration inducing gene 15 protein MLD Sphingolipid delta 4 desaturase Sphingolipid delta(4) desaturase DES1 |
Images
|
Fig1:
Western blot analysis of MLD on different lysates with Rabbit anti-MLD antibody (HA721830) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling MLD with Rabbit anti-MLD antibody (HA721830) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721830, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-MLD antibody (HA721830) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721830) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-MLD antibody (HA721830) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721830) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MLD antibody (HA721830) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721830) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.