Human IL-12B p40 Recombinant Rabbit Monoclonal Antibody [PSH02-57]
cat.: HA721834
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL12B/P40 aa 23-328 (P29460). |
| Positive control: | Recombinant IL-12B p40 protein (HA210655). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-58] to Human IL-12B p40 (Detector) (HA721835) and recombinant standard human 12B p40 (HA210655) as the standard. The reference range value is 30.9-2500pg/ml. |
| Uniprot #: | SwissProt: P29460 Human |
| Alternative names: | CLMF CLMF p40 CLMF2 Cytotoxic lymphocyte maturation factor 40 kDa subunit IL 12 subunit p40 IL 12B IL-12 subunit p40 IL-12B IL12 p40 IL12 subunit p40 IL12B IL12B_HUMAN interleukin 12 beta chain Interleukin 12 p40 Interleukin 12 subunit beta Interleukin 12B Interleukin-12 subunit beta natural killer cell stimulatory factor 40 kD subunit NK cell stimulatory factor chain 2 NKSF NKSF2 |
Images
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Fig1:
Sandwich ELISA analysis of human IL-12B p40 matched pair antibodies. Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721834) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-12B p40 protein (HA210655) starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH02-58] Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.