Human VE Cadherin Recombinant Rabbit Monoclonal Antibody [PSH02-62]
cat.: HA721839
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VE-cadherin aa 48-599 (P33151). |
| Positive control: | Recombinant Human VE Cadherin protein (HA210635). |
| Subcellular location: | Cell junction. Cell membrane. Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-61] to Human VE Cadherin (Capture) (HA721838) and recombinant standard Human VE Cadherin (HA210635) as the standard. The reference range value is 0.63-153.09 ng/ml. |
| Uniprot #: | SwissProt: P33151 Human |
| Alternative names: | 7B 4 7B4 7B4 antigen CADH5_HUMAN Cadherin 5 Cadherin 5 type 2 Cadherin 5, type 2 (vascular endothelium) Cadherin 5, type 2, VE cadherin (vascular epithelium) cadherin, vascular endothelial cadherin, vascular endothelial, 1 Cadherin-5 Cadherin5 CD 144 CD144 CD144 antigen CDH 5 CDH5 CDH5 protein Endothelial specific cadherin FLJ17376 OTTHUMP00000174777 Vascular endothelial cadherin Vascular epithelium cadherin VE Cad VE-cadherin VEC |
Images
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Fig1:
Sandwich ELISA analysis of human Cadherin-5 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA721838) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Cadherin-5 protein (HA210635) starting from 20000 pg/ml to 0 pg/ml and detect antibody [PSH02-62] -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.