HA721841

Tropomyosin 2 Recombinant Rabbit Monoclonal Antibody [PSH02-63]

cat.: HA721841

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH02-63

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 33 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Tropomyosin 2 aa 200-250 / 284.
Positive control:	U-2 OS cell lysate, Saos-2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human heart tissue, mouse smooth muscle tissue lysate, human striated muscle tissue, mouse skeletal muscle tissue.
Subcellular location:	Cytoplasm, cytoskeleton.
Recommended Dilutions: WB IHC-P IF-Tissue	1:1,000-1:2,000 1:1,000 1:200
Uniprot #:	SwissProt: P07951 Human \| P58774 Mouse \| P58775 Rat
Alternative names:	Alpha tropomyosin AMCD1 Arthrogryposis multiplex congenital distal type 1 Beta tropomyosin Beta-tropomyosin Cytoskeletal tropomyosin TM30 DA1 DA2B epididymis secretory protein Li 273 FLJ41118 Heat stable cytoskeletal protein 30 kDa HEL-S-273 hscp30 HTM alpha hTM5 MGC14582 MGC3261 MGC72094 NEM1 NEM4 Nemaline myopathy type 4 OK/SW cl.5 Sarcomeric tropomyosin kappa TM 5 TM3 TM30 TM30nm TMSA TMSB TPM 1 TPM 3 TPM1 alpha TPM1 kappa TPM2 TPM2_HUMAN TRK Tropomyosin 1 alpha Tropomyosin 1 alpha chain Tropomyosin 1 alpha chain isoform 6 Tropomyosin 2 (beta) Tropomyosin 2 Tropomyosin 3 Tropomyosin alpha 3 chain Tropomyosin alpha striated muscle isoform Tropomyosin beta chain Tropomyosin gamma Tropomyosin skeletal muscle beta Tropomyosin-2

Images

Fig1: Western blot analysis of Tropomyosin 2 on different lysates with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: U-2 OS cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: Mouse skeletal muscle tissue lysate
Lane 6: Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 37 kDa

Exposure time: 3 minutes;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721841) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Tropomyosin 2 on different lysates with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/2,000 dilution.

Lane 1: Saos-2-si NT cell lysate
Lane 2: Saos-2-si Tropomyosin 2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 37 kDa

Exposure time: 2 minutes 15 seconds;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721841) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721841) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721841) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embeddedrat rat skeletal muscle tissue with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721841) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunofluorescence analysis of paraffin-embedded human striated muscle tissue labeling Tropomyosin 2 with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721841, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig8: Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle tissue labeling Tropomyosin 2 with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721841, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.